nf-core/stableexpression
This pipeline is designed to identify the most stable genes in one or more expression datasets (RNA-seq / Microarray). The ideal use case is for determining the most suitable RT-qPCR reference genes for a given species.
expressionhousekeeping-genesqpcr-analysis
Version history
Added
- #58 Tries to fetch genome annotation from the Ensembl organisms FTP, in addition to Ensembl and NCBI.
Fixed
- #38 Fix bug with species having multiple entries in
g:Profiler(likecanis lupus). Now queryingg:Profilerbeforehand to get the exact list of available species - #37 Fix too short time limit for
IMPUTE_MISSING_VALUESmodule, which resulting in module failture for species comprising a large number of datasets, likehomo sapiens,mus musculus, orarabidopsis thaliana - #36 Check correspondance between Ensembl gene IDs obtained from
g:Profilerand gene IDs contained in the downloaded genome annotation. Now multiple annotations are downloaded and the one displaying the greatest number of matching gene IDs is chosen. If no annotation displayed matching gene IDs, the module tries with NCBI genome annotation. - #61 Fixed an issue with the R Bioconductor ExpressionAtlas package resulting in a bug when trying to download some experiments (for example
Musa acuminata).
Changed
Initial release of the nf-core/stableexpression pipeline.
The full commit history is available here.